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odyssey clx system  (LI-COR)


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    Structured Review

    LI-COR odyssey clx system
    Odyssey Clx System, supplied by LI-COR, used in various techniques. Bioz Stars score: 98/100, based on 615 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/systems+analysis+software/Odyssey+CLx+Image+Studio+Analysis+Software+-+In-Cell+Western+Add-On/pmc13053694-234-6-9
    Average 98 stars, based on 615 article reviews
    odyssey clx system - by Bioz Stars, 2026-11
    98/100 stars

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    Related Articles

    Software:

    Article Title: mTORC1 activity oscillates throughout the cell cycle, promoting mitotic entry and differentially influencing autophagy induction.
    Article Snippet: Ba/F3 (DSMZ #ACC-300) cells were grown in RPMI-1640 (Corning #10-040-CV) with 10% heat-inactivated fetal bovine .. REAGENT or RESOURCE SOURCE IDENTIFIER AZD1775 MedChemExpress Cat #HY-10993 LY2603618 Selleckchem Cat #S2626 Bafilomycin A1 MedChemExpress Cat #HY-100558 Ro-3306 APExBIO Cat #A8885 EGF Gibco Cat #PHG0314 IL-3 R&D Systems Cat #403-ML-010 Experimental models: Cell lines HeLa ATCC Cat #CRM-CCL-2; RRID:CVCL_0030 sgCtl HeLa Provided by Issam Ben-Sahra Lab Villa et al., 202147 sgTSC2 HeLa Provided by Issam Ben-Sahra Lab Villa et al., 202147 U2OS ATCC Cat #HTB-96; RRID:CVCL_0042 Ba/F3 DSMZ Cat #ACC-300; RRID:CVCL_0161 Primary human dermal fibroblasts Cell Applications Cat #106-05n Software ImageJ National Institutes of Health48 https://imagej.nih.gov/ij/ ZenBlue 3.3 Zeiss https://www.zeiss.com/microscopy/en/ products/software/zeiss-zen.html HCS Studio 5.0 Cell Analysis ThermoFisher Scientific Prism 10.0 GraphPad https://www.graphpad.com/ Kaluza 2.1 Backman Coulter https://www.beckman.com/flow- cytometry/software/kaluza Other EBSS ThermoFisher Scientific Cat #24010-043 Normal Goal Serum Normal Goat Serum Cat #5425 DMEM Corning Cat #10-017-CV Fetal Bovine Serum ThermoFisher Scientific Cat #A5256701 Intercept Blocking Buffer Licor Cat #927-70001 Cell Reports 43, 114543, August 27, 2024 17 serum (ThermoFisher Scientific #A5256701), 1% penicillin-streptomycin (Corning #30-002-CI), and 10 ng/mL IL-3 (R&D systems #403-ML-010). .. For double thymidine block, cells were treated with 2 mM thymidine (Sigma #T1895) for 18 h, then released by washing twice with PBS and incubating in complete media (without thymidine) for 8 h. The 2nd thymidine block (2 mM) was then added for another 18 h before release and collection at time points indicated in the figure legends.

    Cell Analysis:

    Article Title: mTORC1 activity oscillates throughout the cell cycle, promoting mitotic entry and differentially influencing autophagy induction.
    Article Snippet: Ba/F3 (DSMZ #ACC-300) cells were grown in RPMI-1640 (Corning #10-040-CV) with 10% heat-inactivated fetal bovine .. REAGENT or RESOURCE SOURCE IDENTIFIER AZD1775 MedChemExpress Cat #HY-10993 LY2603618 Selleckchem Cat #S2626 Bafilomycin A1 MedChemExpress Cat #HY-100558 Ro-3306 APExBIO Cat #A8885 EGF Gibco Cat #PHG0314 IL-3 R&D Systems Cat #403-ML-010 Experimental models: Cell lines HeLa ATCC Cat #CRM-CCL-2; RRID:CVCL_0030 sgCtl HeLa Provided by Issam Ben-Sahra Lab Villa et al., 202147 sgTSC2 HeLa Provided by Issam Ben-Sahra Lab Villa et al., 202147 U2OS ATCC Cat #HTB-96; RRID:CVCL_0042 Ba/F3 DSMZ Cat #ACC-300; RRID:CVCL_0161 Primary human dermal fibroblasts Cell Applications Cat #106-05n Software ImageJ National Institutes of Health48 https://imagej.nih.gov/ij/ ZenBlue 3.3 Zeiss https://www.zeiss.com/microscopy/en/ products/software/zeiss-zen.html HCS Studio 5.0 Cell Analysis ThermoFisher Scientific Prism 10.0 GraphPad https://www.graphpad.com/ Kaluza 2.1 Backman Coulter https://www.beckman.com/flow- cytometry/software/kaluza Other EBSS ThermoFisher Scientific Cat #24010-043 Normal Goal Serum Normal Goat Serum Cat #5425 DMEM Corning Cat #10-017-CV Fetal Bovine Serum ThermoFisher Scientific Cat #A5256701 Intercept Blocking Buffer Licor Cat #927-70001 Cell Reports 43, 114543, August 27, 2024 17 serum (ThermoFisher Scientific #A5256701), 1% penicillin-streptomycin (Corning #30-002-CI), and 10 ng/mL IL-3 (R&D systems #403-ML-010). .. For double thymidine block, cells were treated with 2 mM thymidine (Sigma #T1895) for 18 h, then released by washing twice with PBS and incubating in complete media (without thymidine) for 8 h. The 2nd thymidine block (2 mM) was then added for another 18 h before release and collection at time points indicated in the figure legends.

    Cytometry:

    Article Title: mTORC1 activity oscillates throughout the cell cycle, promoting mitotic entry and differentially influencing autophagy induction.
    Article Snippet: Ba/F3 (DSMZ #ACC-300) cells were grown in RPMI-1640 (Corning #10-040-CV) with 10% heat-inactivated fetal bovine .. REAGENT or RESOURCE SOURCE IDENTIFIER AZD1775 MedChemExpress Cat #HY-10993 LY2603618 Selleckchem Cat #S2626 Bafilomycin A1 MedChemExpress Cat #HY-100558 Ro-3306 APExBIO Cat #A8885 EGF Gibco Cat #PHG0314 IL-3 R&D Systems Cat #403-ML-010 Experimental models: Cell lines HeLa ATCC Cat #CRM-CCL-2; RRID:CVCL_0030 sgCtl HeLa Provided by Issam Ben-Sahra Lab Villa et al., 202147 sgTSC2 HeLa Provided by Issam Ben-Sahra Lab Villa et al., 202147 U2OS ATCC Cat #HTB-96; RRID:CVCL_0042 Ba/F3 DSMZ Cat #ACC-300; RRID:CVCL_0161 Primary human dermal fibroblasts Cell Applications Cat #106-05n Software ImageJ National Institutes of Health48 https://imagej.nih.gov/ij/ ZenBlue 3.3 Zeiss https://www.zeiss.com/microscopy/en/ products/software/zeiss-zen.html HCS Studio 5.0 Cell Analysis ThermoFisher Scientific Prism 10.0 GraphPad https://www.graphpad.com/ Kaluza 2.1 Backman Coulter https://www.beckman.com/flow- cytometry/software/kaluza Other EBSS ThermoFisher Scientific Cat #24010-043 Normal Goal Serum Normal Goat Serum Cat #5425 DMEM Corning Cat #10-017-CV Fetal Bovine Serum ThermoFisher Scientific Cat #A5256701 Intercept Blocking Buffer Licor Cat #927-70001 Cell Reports 43, 114543, August 27, 2024 17 serum (ThermoFisher Scientific #A5256701), 1% penicillin-streptomycin (Corning #30-002-CI), and 10 ng/mL IL-3 (R&D systems #403-ML-010). .. For double thymidine block, cells were treated with 2 mM thymidine (Sigma #T1895) for 18 h, then released by washing twice with PBS and incubating in complete media (without thymidine) for 8 h. The 2nd thymidine block (2 mM) was then added for another 18 h before release and collection at time points indicated in the figure legends.

    Blocking Assay:

    Article Title: mTORC1 activity oscillates throughout the cell cycle, promoting mitotic entry and differentially influencing autophagy induction.
    Article Snippet: Ba/F3 (DSMZ #ACC-300) cells were grown in RPMI-1640 (Corning #10-040-CV) with 10% heat-inactivated fetal bovine .. REAGENT or RESOURCE SOURCE IDENTIFIER AZD1775 MedChemExpress Cat #HY-10993 LY2603618 Selleckchem Cat #S2626 Bafilomycin A1 MedChemExpress Cat #HY-100558 Ro-3306 APExBIO Cat #A8885 EGF Gibco Cat #PHG0314 IL-3 R&D Systems Cat #403-ML-010 Experimental models: Cell lines HeLa ATCC Cat #CRM-CCL-2; RRID:CVCL_0030 sgCtl HeLa Provided by Issam Ben-Sahra Lab Villa et al., 202147 sgTSC2 HeLa Provided by Issam Ben-Sahra Lab Villa et al., 202147 U2OS ATCC Cat #HTB-96; RRID:CVCL_0042 Ba/F3 DSMZ Cat #ACC-300; RRID:CVCL_0161 Primary human dermal fibroblasts Cell Applications Cat #106-05n Software ImageJ National Institutes of Health48 https://imagej.nih.gov/ij/ ZenBlue 3.3 Zeiss https://www.zeiss.com/microscopy/en/ products/software/zeiss-zen.html HCS Studio 5.0 Cell Analysis ThermoFisher Scientific Prism 10.0 GraphPad https://www.graphpad.com/ Kaluza 2.1 Backman Coulter https://www.beckman.com/flow- cytometry/software/kaluza Other EBSS ThermoFisher Scientific Cat #24010-043 Normal Goal Serum Normal Goat Serum Cat #5425 DMEM Corning Cat #10-017-CV Fetal Bovine Serum ThermoFisher Scientific Cat #A5256701 Intercept Blocking Buffer Licor Cat #927-70001 Cell Reports 43, 114543, August 27, 2024 17 serum (ThermoFisher Scientific #A5256701), 1% penicillin-streptomycin (Corning #30-002-CI), and 10 ng/mL IL-3 (R&D systems #403-ML-010). .. For double thymidine block, cells were treated with 2 mM thymidine (Sigma #T1895) for 18 h, then released by washing twice with PBS and incubating in complete media (without thymidine) for 8 h. The 2nd thymidine block (2 mM) was then added for another 18 h before release and collection at time points indicated in the figure legends.



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